Jes Rejoice Paul
American University of St Vincent, USA
Title: Dopamine D1 and D2 receptor subtypes functional regulation in unilateral rotenone lesioned parkinson’s rat model: effect of serotonin, dopamine and norepinephrine
Biography
Biography: Jes Rejoice Paul
Abstract
Parkinson’s disease (PD) is due to widespread degeneration in the central and peripheral nervous systems. Th e hallmark pathology remains in the dopaminergic striatal insuffi ciency and degeneration of dopaminergic neurons in the substantia nigra (SN).
Objectives: - The present study analysed the eff ect of serotonin (5-HT), dopamine and norepinephrine (NE) as treatment on
rotenone induced hemi-Parkinson’s disease in rats and its role in the regulation of Dopamine receptor subtypes in the Corpus Straiatum (CS) of the experimental rats.
Methods: - Unilateral stereotaxic single dose infusions of rotenone were administered to the substantia nigra of adult male Wistar rats. Neurotransmitters –serotonin (5-HT), dopamine and norepinephrine (NE) treatments were given to rotenone induced hemi-Parkinson’s rats. Dopamine receptor and its subtypes (D1 and D2) binding assay were done. Gene expression studies of Dopamine D1 and D2 were done using real-time PCR.
Results: - Scatchard analysis of Dopamine and Dopamine D2 receptor showed a signifi cant increase (p<0.001) and Dopamine D1 receptor showed a signifi cant decrease (p<0.001) in the Bmax in Corpus Striatum of the PD rats compared to control. These altered parameters were reversed to near control in the serotonin and norepinephrine treated Parkinson’s disease rats and no change was observed in Dopamine treated Parkinson’s disease rats. Real-time PCR results confi rmed the receptor data.
Conclusion: - Our results showed serotonin and norepinephrine functionally reversed in Dopamine receptors in rotenone induced hemi-Parkinson’s rat. This has clinical signifi cance in the therapeutic management of Parkinson’s disease.